We conducted this experiments to find out the possibility that DNA patterns obtained by flow cytometry in patients with cervical cancer can be used as a biological prognostic factors.
We studied 31 cervical cancer patients with clinical stage I or II who visited the Clinic of Cervical Neoplasia at Department of Obstetrics and Gynecology, Catholic University Medical College from Jan. 1991 to Dec. 1991, for whom radical
hysterectomy
were performed.
We obtained following results from those patients by analyzing the results and factors such as patterns of c-myc DNA amplification, detection of Human papillomavirus (HPV) subtype, DNA analysis obtained from flow cytometry, clinical stage, age,
histologic findings.
1. The incidence of c-myc DNA amplification was significantly higher in group with lymphovascular involvement than those group without lymphovascular involvement, but the amplification rate of c-myc DNA was not affected by age, clinical stage,
cell
type, histologic differentiation, tumor size, depth of invasion, and pelvic lymph node metastasis.
2. DNA index was significantly higher in non-keratinizing group than that of zing group, but the mean S-phase rate was not significantly different between two groups. The value of DNA index was not significantly affected by other pathologic
characteristics.
3. There was no significant difference in DNA index between the group with c-myc DNA amplification and group without c-myc DNA amplification.
4. The incidence rate of aneuploid and diploid tumors was irrespective of above prognostic factors classified by clinicopathologic characteristics.
5. The group which consisted of 6 cases(19.4%) negative c-myc DNA amplification and diploidy was significantly lower than other three groups which consisted of 25 cases (80.6%), but the ploidy patterns was irrelevant of either presence or absence
of
c-myc DNA amplification.
6. All 5 cases with detection of HPV subtype 16 showed c-myc DNA amplification patterns, of which 3 cases showed aneuploid and 2 cases showed diploid patterns.
7. From these results, we concluded that amplification pattern of c-myc DNA and ploidy patterns of cervical cancer can be used as a index which reflex the clinicopathologic characteristics of cervical cancer, but further follow up observation and
study
over a long period will be needed to use them as a prognostic factors of cervical cancer.
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